HGH¶
Identification¶
| Property | Value |
|---|---|
| CAS Number | 12629-01-5 |
| Molecular Formula | C₉₉₀H₁₅₂₈N₂₆₂O₃₀₀S₇ |
| Molecular Weight | ~22,125 Da |
| Sequence | 191-amino acid polypeptide with 2 disulfide bonds (Cys⁵³-Cys¹⁶⁵, Cys¹⁸²-Cys¹⁸⁹) |
| Appearance | White to off-white lyophilized powder |
| Purity Standard | ≥99% by HPLC |
| Solubility | Sterile water or bacteriostatic water; gentle swirling |
| Storage | 2–8°C (lyophilized); use promptly after reconstitution |
Category: Muscle Growth
Catalog SKU: PSH-HGH-100IU
Research Background¶
Human Growth Hormone is a 191-amino acid, single-chain polypeptide synthesized by somatotroph cells in the anterior pituitary. It is the primary regulator of postnatal somatic growth and a key metabolic hormone — controlling the GH/IGF-1 axis via hypothalamic GHRH (+) and somatostatin (-) regulation.
Key Mechanisms¶
| Mechanism | Details |
|---|---|
| JAK-STAT Signaling | GH→GHR dimerization→JAK2→STAT5→IGF-1 transcription |
| IGF-1 Cascade | Hepatic IGF-1 production → systemic anabolic and growth effects |
| Lipolysis | Direct GH receptor activation on adipocytes → enhanced fat mobilization |
| Protein Synthesis | GH + IGF-1 → amino acid uptake and protein synthesis in skeletal muscle |
Research Focus Areas¶
Body Composition¶
GH effects on lean mass, fat mass, bone density
Muscle Protein¶
Anabolic signaling; fractional synthetic rate
Aging Research¶
Age-related GH decline (somatopause); IGF-1 models
Metabolism¶
Lipolytic effects; insulin sensitivity modulation
Recovery & Repair¶
GH effects on tissue repair; collagen synthesis
Available Configurations¶
| Configuration | Content | Best For |
|---|---|---|
| 100 IU Kit | 10 IU (~3.3mg) × 10 vials | Evaluation; small-scale research |
| 150 IU Kit | 15 IU (~5mg) × 10 vials | Medium-scale protocols |
| 200 IU Kit | 20 IU (~6.7mg) × 10 vials | High-throughput; bulk research |
| Custom | Custom IU × custom vials | OEM; specific protocols |
Tiered Wholesale Pricing¶
| Volume | Discount | For |
|---|---|---|
| 1 kit | List price | Evaluation; competitive analysis |
| 5+ kits | 10% off | Initial portfolio expansion |
| 20+ kits | 20% off | Mid-size distributors |
| 50+ kits | 30% off | Regional distributors |
Quality Specifications¶
| Parameter | Method | Criterion |
|---|---|---|
| Purity (HPLC) | RP-HPLC, C18 | ≥99.0% peak area |
| Identity (MW) | ESI-MS / MALDI-TOF | ~22,125 ± 5.0 Da |
| Appearance | Visual | White to off-white powder |
| Protein Content | UV A₂₈₀ / Bradford | ≥90% of labeled content |
| Purity (SDS-PAGE) | Reducing SDS-PAGE | Single band at ~22 kDa (bulk orders) |
| Residual Moisture | Karl Fischer | ≤3% |
| Endotoxin | LAL assay | ≤1.0 EU/mg (on request) |
Frequently Asked Questions¶
Q1: What's the difference between HGH and GHS peptides (CJC-1295, Ipamorelin)?¶
HGH directly activates GH receptors on target tissues and stimulates IGF-1. GHS peptides (CJC-1295, Ipamorelin) stimulate pituitary release of endogenous GH. For direct GH receptor studies: use HGH. For GH axis regulation studies: use GHS peptides. In body composition research, HGH gives precise dose control; GHS peptides produce more physiological but less controllable responses.
Q2: How should HGH be reconstituted and stored?¶
Reconstitute with sterile or bacteriostatic water — add diluent gently down vial wall, don't squirt onto powder. Swirl gently (never shake or vortex — denatures protein). Store reconstituted at 2–8°C, use within 7–14 days. Never freeze-thaw — causes aggregation and bioactivity loss.
Q3: Which kit size should I order?¶
Rodent research: 100 IU kit (10 IU vials) for weight-based dosing. Larger animal models or high-concentration cell culture: 150 IU or 200 IU kits. Uncertain? Start with 100 IU evaluation. Contact us — share your model and we'll recommend the right configuration.
Q4: How do you verify it's actually intact HGH and not a fragment?¶
Three methods: (1) Mass spectrometry confirms MW ~22,125 Da ±5 Da — a fragment would show different mass. (2) SDS-PAGE shows single band at ~22 kDa — multiple bands = impurities or degradation. (3) RP-HPLC confirms ≥99% single-peak purity. All three in our QC release testing for research-grade HGH.
Key Research References¶
| Reference | PMID | Key Finding |
|---|---|---|
| Rudman D et al. "Effects of human growth hormone in men over 60 years old." N Engl J Med. 1990;323(1):1–6. | 2355952 | Landmark study demonstrating that recombinant hGH administration increased lean body mass and decreased adipose mass in elderly men; foundational work on GH/IGF-1 axis in aging |
| Yarasheski KE et al. "Effect of growth hormone and resistance exercise on muscle growth in young men." Am J Physiol. 1992;262(3 Pt 1):E261–E267. | 1550219 | hGH combined with resistance exercise increased muscle protein synthesis; characterisation of JAK-STAT/IGF-1 anabolic pathway in skeletal muscle |
Stability & Storage¶
| Condition | Degradation Profile |
|---|---|
| Lyophilized Storage | 24 months at 2–8°C; protect from light and moisture. 191-amino acid protein — more sensitive than small peptides |
| Reconstitution | Use sterile or bacteriostatic water. Add diluent gently down vial wall. Swirl gently — NEVER shake or vortex (denatures the protein) |
| Aggregation | hGH is aggregation-prone. Never freeze-thaw reconstituted hGH — causes irreversible aggregation and bioactivity loss |
| Reconstituted Storage | 2–8°C only; use within 7–14 days. Do NOT freeze reconstituted solutions |
| pH Sensitivity | Optimal stability at pH 6.8–7.4. pH extremes cause denaturation and precipitation |
Source & Purchase¶
For researchers requiring HGH with full analytical documentation including HPLC and LC-MS traces, visit the PeptideSourceHub product page for bulk pricing and specifications.
Related Products¶
| Product | Link |
|---|---|
| CJC-1295 (DAC) | cjc-1295-dac.md — Long-acting GHRH analogue |
| CJC-1295 (no DAC) | cjc-1295-no-dac.md — Pulsatile GHRH analogue |
| Ipamorelin | ipamorelin.md — Selective GHS-R1a agonist |
| PEG-MGF | peg-mgf.md — PEGylated IGF-1Ec for satellite cell activation |